Anti-GP2 Rabbit Polyclonal Antibody for WB, ELISA - P55259

Core Product Specifications and Parameters

Biological Background: GP2 Function and Localization

• GP2, also known as pancreatic secretory granule membrane major glycoprotein or ZAP75, is a ~59 kDa protein encoded by the GP2 gene (Gene ID: 2813) in humans, with a predicted molecular weight of 59480 Da according to UniProt.
• It functions as an intestinal M-cell transcytotic receptor specific for type-I-piliated bacteria, binding fimH on the bacterial pilus tip and internalizing bacteria such as E. coli and S. typhimurium to elicit mucosal immune responses.
• The protein is expressed in the pancreas and specifically in intestinal M cells, as demonstrated at the protein level. Related references: PMID:10760606, PMID:8666297, PMID:19907495
• Subcellularly, GP2 localizes to the zymogen granule membrane, apical cell membrane, membrane rafts, and endosomes; it is also secreted.
• Structurally, GP2 contains an EGF-like domain, a GPI anchor, and disulfide bonds, and it undergoes alternative splicing and N-linked glycosylation.
• GP2 plays a crucial role in innate immunity by mediating transcytosis of pathogens across the intestinal barrier, facilitating antigen delivery to underlying lymphoid follicles.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 111–387 of human GP2, which includes extracellular regions potentially involved in ligand binding; this design may generate antibodies recognizing native conformations and functional epitopes.
• For Western blotting, consider testing whole cell lysates from pancreatic or intestinal tissues known to express GP2; a band near 59 kDa is expected, but glycosylation or other modifications may alter migration.
• Cross-reactivity with mouse and rat GP2 has been confirmed; when validating in these species, ensure antibody specificity with appropriate controls (e.g., peptide blocking or knockout cells).
• In ELISA applications, the recombinant immunogen can serve as a positive control or standard; optimize coating buffer and detection conditions for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined path to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With this anti-GP2 rabbit polyclonal antibody, we support mucosal immunity and intestinal M-cell research, facilitating projects from target discovery to assay development. Tell us your requirements, and we'll help you source specialized reagents—even for rare or emerging targets—so you can accelerate from concept to clinic.