Anti-GOT2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P00505

Core Product Specifications and Parameters

Biological Background: GOT2 Function and Localization

• GOT2 encodes mitochondrial aspartate aminotransferase, also known as kynurenine aminotransferase 4 (KAT4), fatty acid-binding protein (FABP-1), and plasma membrane-associated fatty acid-binding protein (FABPpm).
• Catalyzes the irreversible transamination of L-kynurenine to produce kynurenic acid, an endogenous neuroactive metabolite.
• Functions in the malate-aspartate shuttle, playing a critical role in maintaining intracellular NAD(H) redox balance and metabolite exchange between mitochondria and cytosol.
• Facilitates cellular uptake of long-chain free fatty acids, linking amino acid and lipid metabolism.
• Localizes primarily to the mitochondrial matrix and also associates with the cell membrane.
• Undergoes post‑translational modifications including acetylation, phosphorylation, nitration, and methylation, which may regulate its activity.
• Alternative splicing and multiple aliases (e.g., mitAAT, KYAT4) reflect its functional diversity.
• Associated with disease variants linked to developmental and epileptic encephalopathy (DEE82), underscoring its neurological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1–100) of human GOT2; verify that the epitope is not masked by post‑translational modifications or alternative splicing in your sample.
• For Western blot, a band around 48 kDa is expected; validate with appropriate positive and negative controls.
• In immunohistochemistry (IHC‑P), consider antigen retrieval steps and confirm staining patterns in known positive tissues (e.g., liver, kidney) to establish specificity.
• For ELISA, additional optimization (e.g., coating conditions, paired detection antibody) may be required for robust signal.
• Cross‑reactivity with mouse and rat implies the epitope is conserved; however, always confirm reactivity in the relevant species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in connecting diagnostic manufacturers, laboratories, and researchers with high‑quality IVD raw materials, technical services, and consultative support—from concept to clinic. This anti‑GOT2 antibody aligns with metabolic and neuroscience research demands, and our platform offers a full range of complementary reagents, including validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary buffers. Whether you need established catalog products or assistance sourcing rare‑target reagents, CamelBio streamlines procurement and scalability for your assay development.