Anti-GOT1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P17174

Core Product Specifications and Parameters

Biological Background: Aspartate Aminotransferase, Cytoplasmic (GOT1) Function and Localization

• GOT1 (glutamic-oxaloacetic transaminase 1, cytoplasmic) is also known as AST, AST1, SGOT, cCAT, cAspAT, and GIG18. It is a pyridoxal-phosphate-dependent aminotransferase.
• Its primary reaction is the reversible interconversion of L-aspartate and 2-oxoglutarate to oxaloacetate and L-glutamate, linking amino acid and carbohydrate metabolism via the malate-aspartate shuttle.
• In the brain, GOT1 acts as a glutamate scavenger, helping regulate excitatory neurotransmission and contributing to neuroprotection. Related references: PMID:21900944
• Using L-cysteine as a substrate, GOT1 produces mercaptopyruvate, a key precursor for hydrogen sulfide (H2S) synthesis. H2S acts as a synaptic modulator and neuroprotectant. Related references: PMID:21900944 PMID:27827456
• GOT1 also catalyzes the conversion of (2S)-2-aminobutanoate, a metabolic by-product of the cysteine biosynthesis pathway. Related references: PMID:27827456
• In the liver and adipose tissue, GOT1 participates in gluconeogenesis and glyceroneogenesis, respectively, influencing systemic energy homeostasis.
• The enzyme is predominantly localized in the cytoplasm and is subject to phosphorylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 244–413 of human GOT1, a region outside the active site; thus, the antibody may recognize both native and denatured forms of the protein.
• For Western blot (WB), a band at approximately 46 kDa is expected. Consider using whole-cell lysates from tissues rich in GOT1, such as liver or brain, as positive controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), anticipate cytoplasmic staining. Optimize antigen retrieval and permeabilization protocols as needed.
• When performing ELISA, titrate the antibody to determine optimal capture/detection conditions. This antibody may be used in combination with matching antigen or detection antibodies for sandwich ELISA format.
• Given cross-reactivity with mouse and rat, this antibody is suitable for translational studies in these model organisms; validate in the relevant sample system to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider for IVD raw materials, supporting your entire development pipeline from concept to clinic. For your aspartate aminotransferase research and diagnostic assay needs, we supply high-quality antibodies like this anti-GOT1 polyclonal antibody, as well as matched antigen, recombinant proteins, and ancillary reagents. Our sourcing capabilities span bulk orders, customized antibody development, and technical consulting to streamline your transition to validated clinical tests.