Anti-GOSR2 Rabbit Polyclonal Antibody for WB, ELISA - O14653

Core Product Specifications and Parameters

Biological Background: GOSR2 Function and Localization

• GOSR2 (Golgi SNAP receptor complex member 2), also known as membrin, Bos1, GS27, EPM6, and MYOS, is a ~25–27 kDa SNARE protein encoded by the GOSR2 gene on chromosome 17.
• Core function: mediates vesicle docking and fusion during anterograde protein transport from the cis/medial-Golgi to the trans-Golgi network by forming a SNARE complex with syntaxin-5, Bet1, and Sec22b.
• Alternative splicing generates multiple isoforms; the canonical isoform contains a cytosolic SNARE motif, a coiled-coil region, and a C-terminal transmembrane domain anchoring it to Golgi membranes.
• Subcellular localization: predominantly found in the Golgi apparatus membrane, extending to the cis-Golgi network and endoplasmic reticulum membrane, reflecting its role in early secretory pathway trafficking.
• Post-translational modifications include acetylation; the protein is a target for proteomic identification and features a 3D-structure determined by X-ray crystallography.
• Disease-associated mutations (e.g., Gly144Trp) are linked to congenital muscular dystrophy, epilepsy, and neurodegeneration, underscoring its essential role in neuronal Golgi function.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal cytosolic domain (aa 1–190), which includes the SNARE motif. The resulting polyclonal antibody may recognize both the full-length protein and potential cleavage products in western blotting.
• For WB, endogenous GOSR2 can be detected at ~25 kDa in human cell lines (e.g., HeLa, HEK293) or tissue lysates; a membrane-enriched fraction may improve signal.
• For ELISA, plate-coating with recombinant GOSR2 protein or cell lysates is recommended; consider testing cross-reactivity with related SNAREs if specificity is critical.
• Validate the antibody in the relevant sample system, as cross-reactivity with non-human species is not characterized, and performance may vary under different experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with high-quality IVD raw materials, including validated antibodies like this GOSR2 polyclonal antibody, which targets a key Golgi transport protein implicated in muscular dystrophy and neurodegeneration. With extensive capabilities in supplying optimized antibody pairs, bulk reagents, and rare-target sourcing, we help streamline your workflow from concept to clinic. Contact us to discuss tailored sourcing solutions for your next development project.