Anti-GORAB Polyclonal Antibody for WB, ELISA - Q5T7V8

Core Product Specifications and Parameters

Biological Background: GORAB Function and Localization

• GORAB, also known as NTKLBP1 or SCYL1BP1, is a RAB6-interacting golgin that plays a crucial role in the organization and function of the Golgi apparatus, particularly at the trans-Golgi network.
• It is involved in protein post-translational modification, specifically the glycosylation of extracellular matrix proteins, ensuring proper processing and trafficking.
• GORAB cooperates with SCYL1 to promote the assembly of COPI coat structures into discrete functional domains, which is essential for maintaining Golgi morphology and vesicular transport.
• This protein localizes to the cytoplasm and cycle between the cytosol and Golgi stacks, with predominant association with the trans-Golgi network.
• The GORAB gene undergoes alternative splicing, producing isoforms that may have distinct functions; the primary isoform has a calculated molecular weight of approximately 42 kDa.
• Mutations in GORAB are associated with geroderma osteodysplasticum, a condition characterized by dwarfism and osteoporosis, highlighting its importance in connective tissue development.
• As a coiled-coil domain-containing protein, GORAB likely mediates protein-protein interactions necessary for the structural integrity of the Golgi.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 1-100 of human GORAB. For Western blot (WB), a specific band around 42 kDa is expected; additional bands may correspond to isoforms or post-translational modifications.
• Since the antibody cross-reacts with mouse, consider using mouse cell lines or tissues as a positive control, while also validating reactivity in human samples.
• For ELISA applications, direct or indirect coating with the immunogen peptide may serve as a positive control; optimize blocking and detection conditions to minimize background.
• Given the polyclonal nature of this antibody, it may recognize multiple epitopes. Assess lot-to-lot consistency and validate in the relevant sample system before use.
• GORAB is a cytoplasmic and Golgi-resident protein; consider using appropriate lysis buffers and subcellular fractionation to enrich for Golgi if signal is weak.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner offering a comprehensive portfolio including validated antibodies, antigen standards, and ancillary reagents for diagnostic development. For research on Golgi biology, protein trafficking, and glycosylation disorders like geroderma osteodysplasticum, CamelBio can supply this anti-GORAB polyclonal antibody along with customized bulk orders, matched antibody pairs, and rare target sourcing solutions. Let CamelBio accelerate your project from concept to clinic with reliable, quality-assured raw materials and technical support.