Anti-GOLPH4 Monoclonal Antibody for WB, ELISA - O00461

Core Product Specifications and Parameters

Biological Background: GOLPH4 (GOLIM4) Function and Localization

• Golgi integral membrane protein 4 (GOLIM4), also known as GIMPc, GOLPH4, or GPP130, is a type II transmembrane glycoprotein.
• It localizes predominantly to the cis-Golgi stack membrane and also cycles to endosome membranes.
• Functions in protein trafficking from endosomes to the Golgi apparatus, specifically mediating the late endosome-bypass pathway.
• The protein is post-translationally modified by phosphorylation and myristoylation.
• It contains a coiled-coil domain and a signal-anchor transmembrane region.
• Predicted molecular weight is approximately 82 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide matching amino acids 1-100 of human GOLPH4 (O00461), targeting the N-terminal cytoplasmic region. Consider using this antibody in applications requiring detection of native or denatured protein.
• For Western blot (WB), use appropriate human cell lines or tissues expressing GOLPH4; optimize protein extraction for membrane proteins, and validate with appropriate controls.
• For ELISA, ensure proper coating and detection conditions; the antibody may be used as a capture or detection reagent depending on experimental design.
• Since the protein is a Golgi membrane protein, consider fractionation or enrichment techniques to improve signal in WB.
• Always validate cross-reactivity and specificity in the relevant sample system before use in critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, offering validated antibody pairs, recombinant proteins, and ancillary reagents for efficient assay development. Our anti-GOLPH4 antibody supports research into endosome-to-Golgi trafficking, while we also provide bulk monoclonal antibodies and rare-target raw material access to meet your project needs.