Anti-GOLPH3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H4A6

Core Product Specifications and Parameters

Biological Background: GOLPH3 Function and Localization

• GOLPH3 (Golgi phosphoprotein 3), also known as GPP34, MIDAS, or Vps74, is a 34 kDa peripheral membrane protein encoded by the GOLPH3 gene.
• It is a phosphatidylinositol-4-phosphate (PI4P) binding protein that links Golgi membranes to the actin cytoskeleton and may generate tensile force required for vesicle budding, contributing to Golgi architecture and membrane trafficking.
• GOLPH3 is involved in anterograde transport from the Golgi to the plasma membrane and regulates secretion, potentially by interacting with coatomer.
• It also plays a role in the localization of Golgi enzymes through interaction with their cytoplasmic domains.
• Beyond the Golgi, GOLPH3 localizes to the trans-Golgi network, endosomes, the mitochondrial intermembrane space, and the cell membrane.
• GOLPH3 has been implicated in modulating mTOR signaling and in regulating mitochondrial lipid biosynthesis, linking it to cellular metabolism.
• Tissue-specific expression: detected in muscle fibers of patients with mitochondrial diseases but not in normal muscle fibers.
• Keywords: 3D-structure, Cell membrane, Disulfide bond, Endosome, Golgi apparatus, Lipid-binding, Membrane, Mitochondrion, Phosphoprotein, Protein transport, Proteomics identification, Reference proteome, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human GOLPH3 (residues 1-298), suggesting that the antibody recognizes native and denatured forms; consider it for WB under reducing conditions.
• For WB, a band at approximately 34 kDa is expected in human, mouse, and rat samples; include appropriate controls.
• For IF/ICC, expect Golgi and vesicular staining; co-staining with Golgi markers is recommended to confirm specificity.
• ELISA applications may require optimization of coating antigen and dilution factors; validate in your specific assay system.
• Cross-reactivity with mouse and rat is reported, but validate in the relevant sample system if used for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For GOLPH3-related research and diagnostic development, our team can support with tailored solutions from concept to clinic. Contact us for customized reagent sourcing and technical consultation.