Anti-GOLPH2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8NBJ4

Core Product Specifications and Parameters

Biological Background: GOLPH2 Function and Localization

• Golgi membrane protein 1 (GOLM1, also known as GOLPH2, GP73, and HEL46) is a type II single-pass transmembrane protein primarily localized to the cis-Golgi network membrane.
• Although its precise molecular function remains to be fully elucidated, GOLPH2 is characterized as a cellular response protein to viral infection, with significantly upregulated expression in hepatocytes upon hepatitis virus (HBV, HCV) exposure.
• GOLPH2 is widely expressed in human tissues, with high levels in colon, prostate, trachea, and stomach; lower expression is observed in testis, muscle, lymphoid tissues, white blood cells, and spleen, predominantly in cells of the epithelial lineage.
• In the liver, GOLPH2 is constitutively expressed by biliary epithelial cells but is normally low or absent in hepatocytes; its expression in hepatocytes dramatically increases in virally induced liver diseases, serving as a potential biomarker for viral hepatitis and advanced liver disease.
• Elevated hepatocyte GOLPH2 expression is specific to viral causes and is not seen in non-viral liver conditions such as alcohol-induced liver disease or autoimmune hepatitis, highlighting its diagnostic utility.
• The protein undergoes multiple post-translational modifications including glycosylation, phosphorylation, and acetylation, and contains structural features such as a signal-anchor, a transmembrane helix, and a coiled-coil domain (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen (amino acids 112–401) corresponds to the luminal domain of human GOLPH2, which is highly conserved across human, mouse, and rat, explaining the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is approximately 45 kDa; consider using a 10% SDS-PAGE gel and standard transfer conditions, and validate the signal in appropriate positive control lysates (e.g., liver tissue from HBV-infected patients or GOLPH2-transfected cells).
• In immunohistochemistry (IHC-P), antigen retrieval using citrate or EDTA buffer (pH 6.0 or 9.0) may improve staining; test on formalin-fixed paraffin-embedded liver sections from viral hepatitis cases to confirm specificity.
• For immunofluorescence (IF/ICC) and ELISA, ensure that the antibody is titrated and validated against your specific sample types, as GOLPH2 expression can vary with disease state and cell context.
• Always include appropriate controls (e.g., siRNA knockdown or knockout cell lines) to verify antibody specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, offering a comprehensive range of antibodies, ancillary reagents, and custom procurement services to support diagnostic development and research. This GOLPH2 polyclonal antibody is part of our expanding catalog for liver disease biomarkers and Golgi biology, complementing our supply solutions for validated antibody pairs, optimized monoclonal antibodies, and rare target raw materials. Leverage CamelBio’s integrated supply chain to accelerate your projects from concept to clinic.