Anti-Golgin-45 (BLZF1) Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H2G9

Core Product Specifications and Parameters

Biological Background: Golgin-45 Function and Localization

• Golgin-45 (also known as BLZF1, JEM-1, p45 basic leucine-zipper nuclear factor) is a coiled-coil protein that plays a critical role in maintaining Golgi structure and mediating protein transport from the endoplasmic reticulum through the Golgi to the cell surface.
• It localizes predominantly to the Golgi apparatus membrane, with additional presence in the nucleus and cytoplasm, suggesting dynamic shuttling or context-dependent functions.
• The BLZF1 gene generates multiple isoforms via alternative splicing, and the protein undergoes post-translational modifications including phosphorylation, ADP-ribosylation, and ubiquitin-like conjugation.
• Tissue expression analysis indicates specific detection in the adrenal gland. Related references: PMID:9129147
• Functional studies link golgin-45 to the regulation of ER-Golgi transport, a key step in the secretory pathway, making it a valuable marker for Golgi biology research.
• The protein's nuclear/cytoplasmic localization may also hint at roles beyond transport, possibly in transcriptional regulation given its basic leucine zipper domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-400 of human BLZF1; consider optimizing antibody dilution for each application (WB, IF/ICC, ELISA) using standard titration protocols.
• Predicted molecular weight is ~45 kDa; adjust gel electrophoresis conditions accordingly and include appropriate molecular weight markers.
• For IF/ICC, validate fixation and permeabilization conditions in your specific cell line, as Golgi staining may be sensitive to method.
• While the antibody is reported to cross-react with mouse, confirm reactivity in your model system before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with access to validated IVD raw materials, including this polyclonal antibody targeting golgin-45 for studies in intracellular transport and Golgi biology. Our supply network offers optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing capabilities to streamline your development pipeline. Reach out to explore how CamelBio can support your next immunoassay or research project with reliable, high-performance reagents.