Anti-GOLGA5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8TBA6

Core Product Specifications and Parameters

Biological Background: GOLGA5 Function and Localization

• GOLGA5, also known as Golgin-84, RET-fused gene 5 protein, or Cell proliferation-inducing gene 31 protein, is a member of the golgin family featuring coiled-coil domains and a C-terminal transmembrane helix.
• It localizes to the Golgi apparatus membrane and plays a critical role in maintaining Golgi architecture by promoting the formation of stacked cisternae and ribbon-like structures.
• Functionally, GOLGA5 participates in intra-Golgi retrograde transport, facilitating the recycling of resident Golgi enzymes and maintaining organelle homeostasis.
• The protein is subject to various post-translational modifications, including acetylation, methylation, and phosphorylation, as identified by proteomics studies.
• Tissue expression is ubiquitous but markedly enriched in testis (seminiferous tubules and Leydig cells), while being almost absent in spermatozoa—pointing to a regulated expression pattern during spermatogenesis.
• GOLGA5 is associated with chromosomal rearrangements (e.g., RET fusions) and undergoes alternative splicing, producing multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 300 amino acids of human GOLGA5, which includes predicted coiled-coil regions; this design may favor detection of the full-length protein in denaturing and native conditions.
• For Western blot (WB), a band around 83 kDa is expected; ensure adequate membrane blocking and consider a positive control such as HeLa or HEK293 lysates where GOLGA5 is endogenously expressed.
• In immunofluorescence (IF/ICC), anticipate Golgi-like perinuclear staining; co-staining with a Golgi marker (e.g., GM130) can confirm localization specificity.
• The antibody is reported to cross-react with mouse and rat; thus, researchers can validate its performance in these species, but species-specific optimization (e.g., antibody dilution, blocking conditions) is recommended.
• While ELISA reactivity is indicated, it is advisable to assess the antibody’s performance in the specific assay format and coating conditions of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, offering validated raw materials including this anti-GOLGA5 polyclonal antibody. Alongside reliable detection reagents for Golgi and membrane trafficking targets, we provide bulk ancillary reagents, antibody pairs, and custom sourcing to support assay development from early research to clinical validation. Explore our portfolio to streamline your diagnostic pipeline.