Anti-GOLGA4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13439

Core Product Specifications and Parameters

Biological Background: GOLGA4 Function and Localization

• GOLGA4, also known as golgin-245, p230, GCP2, GOLG, CRPF46, and MU-RMS-40.18, is a member of the golgin family, characterized by extensive coiled-coil domains that facilitate tethering and structural roles at the Golgi apparatus.
• Functions in vesicular trafficking at the Golgi, specifically in the delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through interaction with MACF1.
• Involved in endosome-to-Golgi retrograde trafficking, as supported by functional studies (PubMed:29084197). Related references: PMID:29084197
• Localizes predominantly to the cytoplasm and Golgi apparatus membranes, including the trans-Golgi network membrane.
• Post-translational modifications include glycosylation and phosphorylation; the protein is annotated as a phosphoprotein and glycoprotein.
• Contains a conserved GRIP domain that targets it to the trans-Golgi, and its large size (256-261 kDa) contributes to Golgi structural integrity.
• Alternative splicing generates multiple isoforms, which may exhibit tissue-specific or condition-dependent expression patterns.
• Keywords: 3D-structure, Alternative splicing, Coiled coil, Cytoplasm, Glycoprotein, Golgi apparatus, Membrane, Phosphoprotein, Proteomics identification, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-150) of human GOLGA4; this antibody may recognize epitopes conserved across human, mouse, and rat due to high sequence homology in this domain.
• For Western blot, the predicted molecular weight is ~261 kDa; use low-percentage gels and extended transfer times to ensure efficient blotting of high-molecular-weight proteins.
• In IF/ICC, GOLGA4 is membrane-associated; consider optimizing fixation (e.g., methanol or paraformaldehyde) and permeabilization to preserve Golgi morphology.
• While cross-reactivity with mouse and rat is reported, validate in the relevant sample system, especially if using tissues or primary cells, to account for potential species-specific variations.
• ELISA applications can be developed using this antibody; the recombinant immunogen may serve as a positive control for assay validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including high-quality antibodies like this anti-GOLGA4 polyclonal antibody. With expertise in Golgi trafficking and membrane biology research tools, we support your projects from concept to clinic with reliable sourcing, technical consultation, and customized bulk supply options. From validated antibody pairs to ancillary reagents, CamelBio ensures you have the critical components for your assay development and manufacturing needs.