Anti-GOLGA4 Monoclonal Antibody for WB, IHC-P, ELISA - Q13439

Core Product Specifications and Parameters

Biological Background: GOLGA4 Function and Localization

• GOLGA4 belongs to the golgin family of large coiled-coil proteins that are predominantly associated with the Golgi apparatus. It is also known by several alternative names, including 256 kDa golgin, Golgin-245, p230, and Protein 72.1.
• Functions in vesicular trafficking at the Golgi apparatus, facilitating the transport of cargo between Golgi stacks and to/from other organelles.
• Plays a role in the delivery of GPI-linked proteins from the trans-Golgi network (TGN) by interacting with the microtubule-actin cross-linking factor MACF1.
• Involved in endosome-to-Golgi retrograde trafficking, contributing to the recycling and sorting of membrane proteins. Related references: PMID:29084197
• Localizes primarily to the Golgi apparatus membrane, particularly the trans-Golgi network membrane, and is also found in the cytoplasm.
• Subject to post-translational modifications, including phosphorylation and glycosylation, as indicated by UniProt keywords.
• Contains extended coiled-coil domains characteristic of golgins, which mediate protein-protein interactions essential for Golgi structure and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–150) of human GOLGA4, suggesting the antibody may target an epitope within this domain. Verify reactivity with your specific sample system.
• For Western blot, consider that the predicted molecular weight is 261 kDa; use a high-percentage resolving gel (e.g., 6–8%) and optimize transfer conditions (e.g., extended wet transfer) to ensure efficient blotting of this large protein.
• In immunohistochemistry (IHC-P), antigen retrieval and antibody dilution may require optimization. Use a known positive control tissue (e.g., tissues with high Golgi abundance) to validate staining.
• The antibody’s cross-reactivity with mouse and rat allows for studies in rodent models. For immunofluorescence, permeabilization and fixation conditions should be tailored to Golgi membrane proteins.
• Because GOLGA4 functions in vesicular trafficking, co-localization studies with organelle markers (e.g., TGN46, GM130) could provide valuable insight into its dynamic localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive raw-material solutions for IVD development, supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-GOLGA4 mAb, and bulk ancillary reagents. Whether your research focuses on Golgi biology, intracellular trafficking, or cancer diagnostics, CamelBio can source rare targets and offer technical consultation to accelerate your assay development from concept to clinic.