Anti-GOLGA1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q92805

Core Product Specifications and Parameters

Biological Background: GOLGA1 Function and Localization

• GOLGA1, also known as golgin-97, is a member of the golgin family of coiled-coil proteins associated with the Golgi apparatus.
• It plays a key role in vesicular trafficking at the Golgi, particularly in endosome-to-Golgi transport by tethering TBC1D23-bound vesicles to the trans-Golgi network. Related references: PMID:29084197, PMID:38552021
• Selectively regulates E-cadherin transport from the trans-Golgi network in tubulovesicular carriers, influencing cell-cell adhesion. Related references: PMID:34969853
• Subcellular localization: primarily found at the Golgi apparatus membrane, trans-Golgi network membrane, and also in cytoplasmic vesicles like secretory vesicles and acrosomes.
• Contains coiled-coil domains, and is subject to ADP-ribosylation and phosphorylation (UniProt keywords).
• (Microbial infection) Plays a role in poxvirus morphogenesis by translocating to viral factories to facilitate membrane transport for virion assembly.
• The protein has a predicted molecular weight of ~88 kDa (UniProt: 88184 Da).

Experimental Guidance and Technical Tips

• Designed using a recombinant immunogen spanning the N-terminal region (aa 1-300), which is expected to recognize endogenous GOLGA1 in multiple species due to high cross-reactivity with human, mouse, and rat.
• For Western blotting (WB), a band around 88 kDa is expected. Consider optimizing antibody dilution for your specific sample type.
• For immunofluorescence/immunocytochemistry (IF/ICC), GOLGA1 localizes to the Golgi apparatus; a perinuclear, Golgi-like staining pattern should be observed. Co-staining with a Golgi marker is suggested.
• ELISA application can be used for antibody titration or antigen detection; validate in the relevant sample system.
• Store at recommended conditions (shipped on ice bag, likely -20°C for long-term storage). Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material sourcing, delivering validated antibody pairs, optimized polyclonal antibodies like this anti-GOLGA1 product, bulk ancillary reagents, and rare target sourcing for Golgi biology and vesicular trafficking studies. We support diagnostic manufacturers, labs, and research institutes with tailored technical services and consulting to streamline concept-to-clinic development. Contact us to explore how our one-stop supply capabilities can accelerate your project.