Anti-GNT-V/MGAT5 Rabbit Polyclonal Antibody for WB and ELISA - Q09328

Core Product Specifications and Parameters

Biological Background: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Function and Localization

• MGAT5 (GNT-V) is an alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A that belongs to the glycosyltransferase family.
• Catalyzes the addition of N-acetylglucosamine in beta1-6 linkage to alpha-linked mannose of biantennary N-linked oligosaccharides. Related references: PMID:10395745, PMID:30140003
• Essential for the biosynthesis of branched complex-type N-glycans on receptors such as EGFR, TGFR, and CDH2, thereby modulating growth factor signaling. Related references: PMID:10395745, PMID:22614033, PMID:30140003
• Regulates cell adhesion and migration by modifying CDH2 glycosylation, which inhibits homotypic cell-cell adhesion.
• Plays a role in inflammation: MGAT5-dependent TCR glycosylation limits TCR clustering and dampens antigen responses, while it also inhibits monocyte adhesion and diapedesis. Related references: PMID:22614033
• Promotes proliferation of endothelial cells and angiogenesis by facilitating FGF2 release from extracellular matrix.
• Subcellular localization: Golgi apparatus membrane and secreted.
• Post-translational modifications: glycoprotein, contains disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 627-741 of human GNT-V/MGAT5 (NP_002401.1). Consider this region's conservation when expecting cross-reactivity in mouse.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA applications. For WB, an expected band around 85 kDa is observed in human samples; validate in mouse lysates due to cross-reactivity.
• Due to the polyclonal nature, the antibody may detect multiple isoforms or degradation products; optimize the antibody dilution for your specific experimental conditions.
• Pre-validate the antibody in the relevant sample system (e.g., cell lines, tissues) to confirm specificity, especially if studying glycosylation-dependent events.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies like anti-GNT-V/MGAT5 for glycosylation and cell signaling research. Our supply chain covers polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents to support your diagnostic assay development from concept to clinic. For bulk orders, custom formulations, or technical consultation, contact CamelBio to streamline your sourcing needs.