Anti-GNRHR Rabbit Polyclonal Antibody for WB, ELISA - P30968

Core Product Specifications and Parameters

Biological Background: GNRHR Function and Localization

• GNRHR is the receptor for gonadotropin-releasing hormone (GnRH), a key regulator of reproductive function. Alternative names include HH7, GRHR, LRHR, LHRHR, and GNRHR1.
• Upon GnRH binding, GNRHR mediates G-protein coupled signaling, activating a phosphatidylinositol-calcium second messenger system to stimulate secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH).
• The receptor is a member of the G protein-coupled receptor (GPCR) family, featuring seven transmembrane domains, and is subject to alternative splicing, yielding isoform 2 which may inhibit GnRH-R signaling.
• GNRHR is primarily located on the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue expression includes the pituitary gland, as well as ovary, testis, breast, and prostate, but is absent in liver and spleen, highlighting its specific role in reproductive tissues.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for receptor structure and function; mutations in GNRHR are associated with hypogonadotropic hypogonadism.
• The full-length protein has a predicted molecular weight of approximately 37.7 kDa (UniProt), while the product immunogen corresponds to amino acids 1-80 at the N-terminus.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against an N-terminal region (aa 1-80) of human GNRHR; consider using it for detecting both the mature receptor and potentially isoform 2, though validation is recommended.
• For Western blot (WB), the expected band size is around 38 kDa; validate in your specific sample type, as glycosylation may cause a shift.
• ELISA applications may require careful optimization of coating and detection conditions; consider pairing with a monoclonal capture antibody for sandwich ELISA if quantifying soluble receptor fragments.
• Cross-reactivity with mouse and rat is reported; however, sequence homology in the immunogen region should be verified if precise species discrimination is needed.
• Due to membrane protein nature, include proper membrane preparation and solubilization steps in lysis buffers when preparing samples for WB to ensure efficient extraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, and this anti-GNRHR antibody exemplifies our capability in reproductive hormone receptor research tools. We support diagnostic manufacturers and laboratories with validated antibody pairs, monoclonal/polyclonal options, and ancillary reagents for assay development. For rare targets or custom requirements, our sourcing network ensures timely access to high-quality raw materials to accelerate your projects.