Anti-GNRH2 Rabbit Polyclonal Antibody for WB, ELISA - O43555

Core Product Specifications and Parameters

Biological Background: GNRH2 Function and Localization

• GNRH2 encodes progonadoliberin-2, a precursor protein that is processed to produce gonadotropin-releasing hormone II (GnRH II). The gene undergoes alternative splicing, generating multiple isoforms.
• The mature hormone stimulates the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the anterior pituitary, playing a central role in reproductive function.
• GNRH2 expression is highest in the midbrain, but notably it is also expressed at significantly higher levels outside the brain (up to 30-fold), particularly in kidney, bone marrow, and prostate, suggesting broader regulatory roles.
• As a secreted protein, GNRH2 is directed to the secretory pathway via a signal peptide and undergoes post-translational processing, including cleavage on dibasic residues and C-terminal amidation.
• Keywords: hormone, secreted, signal, cleavage on pair of basic residues, amidation, alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 24-120, covering the region beyond the predicted signal peptide and including the mature hormone; this design may detect both precursor and processed forms.
• Predicted molecular weight of the full-length precursor is ~13 kDa; appearance of additional lower molecular weight bands in WB may reflect endogenous processing.
• Validate antibody performance in model systems with confirmed GNRH2 expression, such as human kidney or prostate cell lines, before large-scale experiments.
• The antibody is validated for WB and ELISA; for other applications, in-house optimization and lot-specific validation are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to a wide range of IVD raw materials. For GNRH2 and reproductive hormone studies, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing of rare targets—supporting your project from concept to clinic.