Anti-GNRH1 Polyclonal Antibody for WB, IF/ICC, ELISA - P01148

Core Product Specifications and Parameters

Biological Background: GNRH1 Function and Localization

• Progonadoliberin-1 (GNRH1) is a precursor protein that is processed to produce gonadotropin-releasing hormone 1 (GnRH), a key regulator of the reproductive axis.
• GnRH stimulates the anterior pituitary to secrete luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which are essential for gonadal function.
• The protein is encoded by the GNRH1 gene, also known as GNRH, GRH, and LHRH, located on chromosome 8.
• It is synthesized as a preproprotein with a signal peptide (aa 1-23), and the mature decapeptide (aa 24-33) is released after proteolytic cleavage.
• Subcellularly, GNRH1 is targeted to the secretory pathway and is secreted from hypothalamic neurons.
• Post-translational modifications include amidation of the C-terminus, formation of pyroglutamic acid at the N-terminus, and cleavage at paired basic residues.
• Molecular weight of the unprocessed precursor is approximately 10.38 kDa, but processing yields smaller active peptides.
• Mutations in GNRH1 are associated with hypogonadotropic hypogonadism and Kallmann syndrome, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 24–92, which includes the mature GnRH decapeptide and flanking sequences; thus, the antibody may recognize both precursor and processed forms.
• For Western blot, the predicted molecular weight of the full-length precursor is ~10 kDa; use a high-percentage gel (e.g., 15%) and optimize transfer conditions to retain small peptides.
• Detection of the secreted mature peptide in WB may require concentration of culture supernatant or peptide extraction protocols.
• In IF/ICC, GNRH1 is secreted; consider using Golgi inhibitors (e.g., brefeldin A) to visualize intracellular accumulation in neuroendocrine cells.
• For ELISA, the antibody can be used as capture or detection reagent; validate its performance with recombinant GnRH standards.
• Cross-reactivity with mouse and rat suggests conserved epitopes; however, always verify specificity in the relevant species and tissue type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This GNRH1 antibody supports assay development and research in reproductive endocrinology. For rare-target sourcing and custom logistic support, contact CamelBio's team.