Anti-GNPNAT1 Rabbit Polyclonal Antibody for WB - Q96EK6

Core Product Specifications and Parameters

Biological Background: GNPNAT1 Function and Localization

• GNPNAT1 (Glucosamine 6-phosphate N-acetyltransferase) is also known as phosphoglucosamine acetylase/transacetylase, GNA1, Gpnat1, and RHZDAN.
• It is an acyltransferase that catalyzes the conversion of glucosamine-6-phosphate to N-acetylglucosamine-6-phosphate in the UDP-N-acetylglucosamine biosynthesis pathway.
• Localized primarily to the Golgi apparatus membrane and endosome membrane.
• Human GNPNAT1 gene encodes a protein of approximately 21 kDa (observed), with a calculated molecular weight of ~20.7 kDa.
• The protein is a member of the reference proteome and has been identified via proteomics.
• UniProt keywords highlight its involvement in transferase activity and its 3D structure has been determined.
• Disease variants in GNPNAT1 have been linked to dwarfism (UniProt keyword: Disease variant).

Experimental Guidance and Technical Tips

• Consider using this polyclonal antibody in Western blot under reducing conditions to detect a band at approximately 21 kDa.
• For immunofluorescence, co-stain with Golgi marker (e.g., GM130) to confirm expected localization patterns.
• The Immunogen covers an N-terminal fragment (aa 1–91); ensure appropriate positive controls (e.g., GNPNAT1-overexpressing cells or tissues with high endogenous expression).
• Validate cross-reactivity with mouse and rat samples due to high sequence homology across species.
• Optimize dilution for ELISA applications empirically, as polyclonal antibodies may exhibit lot-to-lot variation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like GNPNAT1—central to UDP-GlcNAc biosynthesis and glycosylation—we offer reliable sourcing of rare raw materials to accelerate assay development. Contact us to streamline your supply chain from concept to clinic.