Anti-GNPDA1 Rabbit Polyclonal Antibody for WB, ELISA - P46926

Core Product Specifications and Parameters

Biological Background: GNPDA1 Function and Localization

• GNPDA1 (glucosamine-6-phosphate deaminase 1) is also known as glucosamine-6-phosphate isomerase 1, oscillin, GNPI, HLN, and KIAA0060. It is encoded by the GNPDA1 gene in humans (Gene ID: 10007).
• Catalyzes the reversible deamination and aldo-keto isomerization of alpha-D-glucosamine 6-phosphate to beta-D-fructose 6-phosphate and ammonium, a key regulatory step in the hexosamine pathway for UDP-GlcNAc biosynthesis. Related references: PMID:21807125 PMID:26887390
• At high ammonium concentrations, drives the amination and isomerization of fructose 6-phosphate toward hexosamines, influencing UDP-GlcNAc synthesis. Related references: PMID:21807125 PMID:26887390
• Plays a role in fine-tuning metabolic fluctuations of cytosolic UDP-GlcNAc and their effects on hyaluronan synthesis during tissue remodeling. Related references: PMID:26887390
• Might trigger calcium oscillations in mammalian eggs, essential for egg activation and early embryonic development (By similarity).
• Localizes to the cytoplasm.
• Belongs to the glucosamine-6-phosphate deaminase family; post-translational modifications include acetylation and phosphorylation as indicated by proteomics.
• Alternative splicing results in multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-70 of human GNPDA1 (N-terminal region). This N-terminal targeting may contribute to the antibody's specificity.
• This antibody is validated for Western blot (WB) and ELISA. For WB, consider using a protein weight of approximately 33 kDa as a reference for GNPDA1.
• Cross-reactivity is reported for human, mouse, and rat; thus, it can be considered for studies in these species, but validation in the specific sample system is recommended.
• For functional studies of the hexosamine pathway or hyaluronan synthesis, this antibody may aid in detecting GNPDA1 expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier providing one-stop access to antibodies, antigens, and technical support from concept to clinic. This GNPDA1 antibody supports research into hexosamine metabolism, tissue remodeling, and related pathological processes. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to meet your diagnostic development needs.