Anti-GNMT Polyclonal Antibody for WB and ELISA - Q14749

Core Product Specifications and Parameters

Biological Background: GNMT Function and Localization

• GNMT (Glycine N-methyltransferase) catalyzes the S-adenosylmethionine (AdoMet)-dependent methylation of glycine to form sarcosine and S-adenosylhomocysteine (AdoHcy). This reaction is regulated by the binding of 5-methyltetrahydrofolate.
• The enzyme is central to methyl group metabolism, tightly controlling the cellular AdoMet/AdoHcy ratio to maintain proper transmethylation activity.
• GNMT is a folate-binding protein and member of the methyltransferase superfamily.
• Subcellular localization is cytoplasmic.
• Tissue expression is largely restricted to the liver, pancreas, and prostate.
• Post-translational modifications include acetylation and phosphorylation; disease-associated variants have been reported.
• The predicted molecular weight based on sequence is 32.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human GNMT protein (aa 1–295). When performing western blotting, consider that the observed band may migrate at approximately 33 kDa, consistent with the reported molecular weight.
• For ELISA applications, ensure appropriate coating and detection conditions are optimized; the polyclonal nature may contribute to signal amplification.
• Due to cross-reactivity with mouse and rat, this antibody may be used for comparative studies across species. Validate in the relevant sample system, particularly liver, pancreas, or prostate tissues where GNMT is expressed.
• As a polyclonal antibody, lot-to-lot consistency should be verified when transitioning between manufacturing batches for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with seamless access to high-quality IVD raw materials. This anti-GNMT polyclonal antibody exemplifies our portfolio for methylation metabolism and liver disease research. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material sourcing—ensuring your projects move efficiently from concept to clinic.