Anti-GNL3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q9BVP2

Core Product Specifications and Parameters

Biological Background: GNL3 Function and Localization

• GNL3, also known as Nucleostemin, E2-induced gene 3 protein (E2IG3), and NNP47, is a nucleolar GTP-binding protein that belongs to the HSR1/MMR1 GTP-binding protein family.
• It may be required to maintain the proliferative capacity of stem cells by stabilizing MDM2 through prevention of its ubiquitination and subsequent proteasomal degradation.
• The protein localizes predominantly to the nucleolus within the nucleus, consistent with its role in ribosome biogenesis and cell cycle regulation.
• Increased expression levels have been observed in lung tissue from cancer patients, suggesting a potential role in tumorigenesis.
• Post-translational modifications include acetylation and phosphorylation; the protein also contains a coiled-coil domain and undergoes alternative splicing, yielding multiple isoforms.
• Keywords associated with GNL3 include GTP-binding, nucleotide-binding, isopeptide bond formation, and Ubl conjugation, highlighting its dynamic regulation and functional versatility.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1–240) of human GNL3, which may contribute to recognition of multiple isoforms; verify specificity in your target sample.
• For Western blotting, a band consistent with the predicted molecular weight of approximately 62kDa is expected; however, post-translational modifications or splicing variants may cause slight shifts.
• In immunoprecipitation experiments, optimize the antibody-to-lysate ratio, keeping in mind the polyclonal nature of this reagent.
• For immunofluorescence/ICC, co-staining with a nucleolar marker is recommended to confirm the expected nuclear and nucleolar staining pattern.
• ELISA performance may require checkerboard titration to determine optimal coating and detection conditions.
• Always include appropriate negative and positive controls, and validate the antibody in the relevant human sample or cell line system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio of IVD raw materials including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This anti-GNL3 antibody supports studies in stem cell biology and cancer research, and CamelBio can assist in scaling from initial development to commercial production. Whether you need custom antibody generation, technical consultation, or reliable supply chain management, CamelBio bridges your concept to the clinic.