Anti-GNGT2 Polyclonal Antibody for WB and ELISA - O14610

Core Product Specifications and Parameters

Biological Background: GNGT2 Function and Localization

• Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-T2, also known as G gamma-C, G-gamma-8, or G-gamma-9, is encoded by the GNGT2 gene (alternative symbols GNG8, GNG9, GNGT8) and belongs to the G protein gamma subunit family.
• As part of heterotrimeric G proteins, it functions as a modulator or transducer in various transmembrane signaling systems; the beta and gamma chains are required for GTPase activity, GDP/GTP exchange, and G protein-effector interaction.
• The protein localizes to the cell membrane.
• Expression is detected in retinal cones, indicating a tissue-specific role in visual signal transduction.
• Post-translational modifications include methylation and prenylation, which are critical for membrane anchoring and protein-protein interactions.
• Keywords associated with GNGT2 encompass cell membrane, lipoprotein, membrane, methylation, prenylation, proteomics identification, reference proteome, and transducer, reflecting its involvement in lipid modification and signaling.
• The mature protein has a predicted molecular weight of approximately 7.7 kDa (UniProt: 7747 Da), consistent with the product specification of ~8 kDa.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering the full-length human GNGT2 sequence (aa 1-69). Consider sequence alignment to predict cross-reactivity with other species or isoforms.
• For Western blot, the predicted molecular weight is approximately 8 kDa. Optimize gel percentage (e.g., high-percentage Tris-tricine gels) and transfer conditions for small proteins.
• The antibody is suitable for ELISA; validate coating buffer, blocking conditions, and detection system in the relevant sample system to achieve optimal signal-to-noise ratio.
• Cross-reactivity with mouse has been reported; verify performance in your specific model system if using other species or tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including high-quality antibodies like this anti-GNGT2 polyclonal antibody, to support signal transduction research and assay development. As a one-stop sourcing partner, we provide validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic projects from concept to clinic.