Anti-GNGT1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P63211

Core Product Specifications and Parameters

Biological Background: GNGT1 Function and Localization

• GNGT1 encodes the gamma-1 transducin subunit, also known as guanine nucleotide-binding protein G(T) subunit gamma-T1.
• It functions as a modulator/transducer in transmembrane signaling systems; together with the beta chain, it is required for GTPase activity, GDP/GTP exchange, and G protein–effector interaction.
• The protein localizes to the cell membrane, consistent with its role in signal transduction.
• Tissue expression is highly specific to retinal rod outer segments, underpinning its involvement in phototransduction.
• Key post-translational modifications include prenylation and methylation, which facilitate membrane anchoring and protein–protein interactions.
• Identified as a lipoprotein and a reference proteome member, highlighting its structural and functional importance in G-protein signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1–45); consider this mapping when interpreting immunoreactivity results.
• For Western blot, due to the low molecular weight (8 kDa), use a high‑concentration acrylamide gel (e.g., 15%) and appropriate low‑range protein markers.
• Validate antibody specificity in the relevant sample system; cross‑reactivity with mouse and rat is predicted based on supplier data.
• In immunofluorescence staining, perform proper permeabilization and include controls, given the membrane localization of the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti‑GNGT1 pAb. For G‑protein signaling research and beyond, we supply bulk ancillary reagents and source rare targets, streamlining your supply chain from concept to clinic.