Anti-GNG7 Rabbit Polyclonal Antibody for WB, ELISA - O60262

Core Product Specifications and Parameters

Biological Background: GNG7 Function and Localization

• Guanine nucleotide-binding proteins (G proteins) are integral modulators and transducers in various transmembrane signaling systems. GNG7 encodes the gamma-7 subunit, which assembles with alpha and beta subunits to form heterotrimeric G proteins.
• The beta-gamma complex (including GNG7) is essential for GTPase activity, GDP/GTP exchange, and effector interactions, enabling G protein-mediated signal transduction.
• GNG7 specifically regulates adenylyl cyclase signaling in the brain, forming a G(olf) subunit alpha-beta-gamma-7 heterotrimer critical for striatal adenylyl cyclase activity (By similarity).
• It localizes primarily to the cell membrane, consistent with its role in transmembrane signaling.
• GNG7 is widely expressed across tissues but is notably down-regulated in pancreatic and esophageal cancers, suggesting a potential role in tumor suppression.
• Post-translational modifications include prenylation, methylation, and acetylation; prenylation is particularly important for membrane anchoring.
• The mature protein has a molecular weight of approximately 8 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-68 amino acids of human GNG7, covering nearly the full-length protein. Consider this when designing peptide blocking experiments or assessing epitope specificity.
• For Western blotting, a band around 8 kDa is expected. Use high-percentage gels (e.g., 15% Tris-glycine) and low-molecular-weight markers. Optimize transfer conditions (e.g., shorter transfer times, 0.2 µm membranes) to retain small proteins.
• Verify cross-reactivity with mouse and rat samples as indicated. Due to sequence conservation, it may also detect GNG7 from other species; validate with endogenous controls.
• For ELISA, use the recombinant immunogen as a positive control and consider indirect or sandwich ELISA formats depending on your assay requirements.
• Always titrate the antibody to determine optimal working concentration for your specific experimental system. Include appropriate negative and positive controls.
• The antibody is supplied as liquid, shipped on ice bags. Store at -20°C upon receipt and avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless procurement solution for IVD raw materials, supporting research and diagnostic development from concept to clinic. This anti-GNG7 polyclonal antibody is part of our extensive portfolio of signal transduction reagents, and we also offer validated antibody pairs, monoclonal antibodies, and ancillary reagents tailored to your project. Contact us for assistance with rare-target sourcing or custom antibody production to accelerate your G protein research and diagnostic innovation.