Anti-GNG10 Rabbit Polyclonal Antibody for WB, ELISA - P50151

Core Product Specifications and Parameters

Biological Background: GNG10 Function and Localization

• Guanine nucleotide-binding protein subunit gamma-10 (GNG10) is a member of the G protein gamma subunit family, which plays a critical role in transmembrane signal transduction by coupling receptors to intracellular effectors.
• As part of the heterotrimeric G protein complex, the beta and gamma subunits are essential for regulating GTPase activity, facilitating the exchange of GDP for GTP, and enabling G protein-effector interactions.
• GNG10 specifically interacts with beta-1 and beta-2 subunits, but not with beta-3, indicating selectivity in G protein assembly and signaling pathways.
• The protein is localized at the cell membrane, where it participates in various transmembrane signaling systems as a modulator or transducer.
• GNG10 is abundantly and ubiquitously expressed across tissues, suggesting a broad functional significance in multiple cell types.
• Post-translational modifications include prenylation, methylation, and acetylation, which are important for membrane association and protein stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-68 of human GNG10, covering nearly the entire protein. This N-terminal region is conserved across human, mouse, and rat, supporting the antibody's cross-reactivity with these species.
• For Western blot, the predicted molecular weight of GNG10 is approximately 7 kDa. Use 15% or gradient SDS-PAGE gels and optimize transfer conditions (e.g., low molecular weight cutoff PVDF membrane, reduced transfer time) to avoid loss of small proteins.
• Given the ubiquitous expression of GNG10, positive signals can be expected in a wide range of human, mouse, and rat cell lysates. However, relative expression levels may vary; validate in your specific model system.
• For ELISA applications, use standard indirect ELISA protocols with recombinant GNG10 protein or cell lysates. Titrate antibody concentrations and include appropriate controls to minimize background.
• Consider performing a no-primary-antibody control to verify specific detection, as polyclonal antibodies may exhibit nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering a wide portfolio of validated antibodies, antigen-grade proteins, and ancillary reagents. Our GNG10 rabbit polyclonal antibody supports G protein signaling research and diagnostic development, and we can further assist with custom monoclonal/polyclonal antibody generation, bulk supply, and sourcing of rare targets to streamline your project from discovery to commercialization.