Anti-GNE Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y223

Core Product Specifications and Parameters

Biological Background: GNE Function and Localization

• GNE encodes a bifunctional enzyme with UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, initiating sialic acid biosynthesis. Related references: PMID:10334995 PMID:11326336 PMID:14707127
• As the rate-limiting enzyme in NeuAc production, GNE governs cell surface sialylation, crucial for cell adhesion, signal transduction, and cellular recognition. Related references: PMID:10334995 PMID:14707127
• Subcellular localization is predominantly cytoplasmic, specifically in the cytosol.
• Highest expression in liver and placenta; also detected in heart, brain, lung, kidney, skeletal muscle, and pancreas. Multiple isoforms show differential tissue distribution.
• Keywords: Allosteric enzyme, alternative splicing, ATP-binding, disease variant (e.g., GNE myopathy), hydrolase, kinase, metal-binding, multifunctional enzyme, nucleotide-binding, phosphoprotein, transferase, zinc.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-150) of human GNE. Verify specificity in your target species (human, mouse, rat) using appropriate positive and negative controls.
• For WB, consider the predicted molecular weight of ~79 kDa and use reducing conditions. Validate band specificity with siRNA knockdown or knockout lysates if available.
• In IHC-P, optimize antigen retrieval and antibody dilution. Include tissue sections with known high expression (e.g., liver, placenta) as positive controls.
• For ELISA, pair with a suitable detection antibody and confirm linearity using recombinant GNE protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement. Whether your research involves sialic acid biosynthesis, cell surface glycosylation, or GNE-related disorders, CAMELBIO ensures reliable access to high-quality reagents to accelerate your diagnostic development from concept to clinic.