Anti-GNB5 Polyclonal Antibody for WB, ELISA - O14775

Core Product Specifications and Parameters

Biological Background: GNB5 Function and Localization

• GNB5 encodes guanine nucleotide-binding protein subunit beta-5 (Gbeta5), a member of the WD repeat protein family involved in signal transduction.
• It enhances GTPase-activating protein (GAP) activity of RGS proteins like RGS7 and RGS9, accelerating GTP hydrolysis on G-alpha subunits to terminate GPCR signaling. Related references: PMID:27677260
• By increasing RGS7 GAP activity, GNB5 regulates mood and cognition.
• Through RGS9 GAP activity enhancement, it contributes to deactivation of D(2) dopamine receptor signaling. Related references: PMID:27677260
• Plays a role in neuronal signaling, particularly in parasympathetic control of heart rate.
• Localized to the membrane.
• Widely expressed across tissues.
• Disease variants are linked to intellectual disability; the protein has been structurally characterized.

Experimental Guidance and Technical Tips

• The antibody was raised against the N-terminal 1–200 amino acids of human GNB5; this region is conserved and may allow detection across species.
• For WB, a band of approximately 44 kDa is expected; loading controls and appropriate sample preparation are recommended.
• Cross-reactivity with mouse and rat indicates potential use in rodent models, but validation in the specific experimental system is advised.
• This polyclonal antibody can be used in ELISA applications; optimizing coating antigen and antibody dilutions is suggested.

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CamelBio provides comprehensive IVD raw material sourcing, offering high-quality antibodies like this anti-GNB5 polyclonal antibody for neuroscience and GPCR research. With capabilities ranging from validated antibody pairs to bulk ancillary reagents and rare target sourcing, CamelBio supports diagnostic manufacturers and researchers from concept to clinic.