Anti-GNB3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P16520

Core Product Specifications and Parameters

Biological Background: GNB3 Function and Localization

• GNB3 encodes the beta-3 subunit of heterotrimeric guanine nucleotide-binding proteins (G proteins), which act as essential transducers in many transmembrane signaling systems.
• The beta subunit forms a stable dimer with a gamma subunit; this complex is critical for enabling GTPase activity of the alpha subunit, facilitating GDP-to-GTP exchange, and interacting with downstream effectors.
• GNB3 is a member of the beta subunit family and is known to associate with alpha subunits of the G(i), G(s), and G(t) classes, thereby directing signals from various G protein-coupled receptors (GPCRs).
• The protein contains WD repeat domains, characteristic of G-beta subunits, which mediate protein-protein interactions essential for signal transduction.
• Alternative splicing of the GNB3 gene produces multiple transcript variants, potentially expanding functional diversity.
• Mutations in GNB3 are linked to congenital stationary night blindness type 1H (CSNB1H), underscoring its role in phototransduction and retinal signaling.

Experimental Guidance and Technical Tips

• The immunogen is designed to cover the full-length human GNB3 protein (amino acids 1–340), which can enable recognition of both native and denatured forms; suitable for WB under standard conditions.
• For WB, the detected band is expected near 37 kDa (predicted molecular weight: 37.2 kDa); including reducing agents in the sample buffer is recommended to achieve a clear band.
• In IF/ICC applications, GNB3 may localize to the plasma membrane or cytoplasm depending on cell type and activation status; optimize fixation and permeabilization conditions accordingly.
• The antibody's reported cross-reactivity with mouse and rat GNB3 suggests conserved epitopes across species; confirm performance in your specific model system.
• For ELISA, both direct and indirect detection protocols can be considered; titrate the antibody to achieve optimal signal-to-noise ratios.
• As a polyclonal antibody, it offers robust signal due to multi-epitope recognition; lot-to-lot consistency should be validated for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to validated raw materials for G protein signaling research, including this anti-GNB3 polyclonal antibody. We supply optimized antibody pairs, bulk ancillary reagents, and support rare target sourcing to streamline your IVD development from concept to clinic.