Anti-GNB2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62879

Core Product Specifications and Parameters

Biological Background: GNB2 Function and Localization

• GNB2 encodes the beta-2 subunit of heterotrimeric guanine nucleotide-binding proteins (G proteins), which function as key modulators and transducers in various transmembrane signaling systems. G proteins relay signals from G protein-coupled receptors (GPCRs) to downstream effectors.
• Together with the gamma subunit, the beta-2 subunit is essential for the GTPase activity of the Gα subunit, facilitates GDP to GTP exchange, and mediates G protein-effector coupling. These interactions are critical for signal propagation and modulation.
• Alternative splicing produces multiple isoforms, and the protein undergoes post-translational modifications such as acetylation and phosphorylation, which may regulate its activity and localization.
• The protein localizes to the cytoplasm, perinuclear region, and cell membrane, consistent with its role in transducing extracellular signals to intracellular compartments.
• GNB2 is widely expressed, with particularly high levels in the brain and all cardiac subcompartments, notably the atrioventricular node, suggesting specialized functions in neural and cardiac signal transmission.
• Mutations in GNB2 are associated with intellectual disability, highlighting its critical role in neurological development and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human GNB2 protein (aa 1-340), which is highly conserved among human, mouse, and rat. This suggests the antibody may recognize the native and denatured forms of GNB2 across these species. Consider validating cross-reactivity in target tissues if required.
• For Western blot (WB), a band at approximately 37 kDa is expected in lysates from human, mouse, or rat cells. Use appropriate positive controls (e.g., brain or cardiac tissue lysates) given the high expression in these tissues.
• For immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), optimal fixation and permeabilization conditions should be determined experimentally. The antibody is expected to show cytoplasmic, perinuclear, and plasma membrane staining based on the protein's localization.
• For ELISA, the antibody can be used as a capture or detection reagent. Note that performance should be validated in the specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This antibody targets GNB2, a key component of G protein signaling, and is suitable for multiple applications in research and diagnostic development. For procurement needs or technical consultation, contact CamelBio.