Anti-GNB2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P62879

Core Product Specifications and Parameters

Biological Background: GNB2 Function and Localization

• GNB2 encodes guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, a critical component of heterotrimeric G proteins that couple seven-transmembrane receptors to intracellular signaling cascades.
• The beta subunit, together with the gamma subunit, is essential for the GTPase activity of the alpha subunit, facilitating the exchange of GDP for GTP and enabling G protein-effector interactions.
• GNB2 belongs to the WD repeat protein family and exhibits alternative splicing, potentially giving rise to different isoforms with distinct functional properties.
• Subcellularly, GNB2 is localized in the cytoplasm, particularly in the perinuclear region, and is associated with the cell membrane where it participates in signal transduction.
• Post-translational modifications, including acetylation and phosphorylation, may regulate GNB2 activity and its interaction with other signaling molecules.
• Tissue expression analysis reveals GNB2 is present in all cardiac subcompartments and in the brain, with highest levels in the atrioventricular node and brain, suggesting a role in cardiac and neurological functions.
• Mutations in GNB2 have been linked to intellectual disability, underscoring its importance in developmental processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-90) of human GNB2, providing specificity for the full-length protein and potentially some isoforms.
• For Western blotting (WB), use reducing and denaturing sample preparation, with an expected band around 37 kDa; validate antibody specificity using positive and negative control lysates.
• In immunohistochemistry (IHC-P), consider antigen retrieval methods and include appropriate tissue controls; cytoplasmic and membrane staining should be assessed in relevant tissues like brain or heart.
• For ELISA, follow standard indirect or sandwich protocols, optimizing antibody concentration and blocking conditions for your specific assay system.
• Cross-reactivity with mouse and rat suggests conserved epitopes; nevertheless, confirm reactivity in the relevant species' sample context.

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