Anti-GNB1L Polyclonal Antibody for WB, ELISA - Q9BYB4

Core Product Specifications and Parameters

Biological Background: GNB1L Function and Localization

• GNB1L, also known as WDR14 and DGCRK3, is a WD repeat-containing protein that functions as a guanine nucleotide-binding protein subunit beta-like protein.
• It acts as a critical regulator of DNA damage response (DDR) signaling by specifically modulating phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins.
• The protein localizes to both the cytoplasm and nucleus, consistent with its role in signal transduction and DNA damage sensing.
• Expression is ubiquitous but enriched in heart, liver, skeletal muscle, kidney, spleen, thymus, and pancreas, with lower levels in lung, placenta, and brain.
• Contains multiple WD40 repeat domains, facilitating protein-protein interactions crucial for its regulatory functions.
• Alternative splicing has been reported, indicating potential isoform diversity.

Experimental Guidance and Technical Tips

• For Western blotting, perform reducing SDS-PAGE and use appropriate loading controls; ensure validation with lysates from human, mouse, or rat tissues/cells given the antibody's cross-reactivity profile.
• When performing ELISA, consider indirect detection and optimize coating antigen concentration and antibody dilution; include relevant positive and negative controls.
• The immunogen corresponds to nearly full-length GNB1L (amino acids 1-327), suggesting recognition of the native protein; however, confirm specificity with knockdown or knockout controls in the target species.
• Note that this antibody has not been validated for applications other than WB and ELISA; additional optimization may be required for alternative techniques.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering this high-quality anti-GNB1L antibody to support DNA damage response research and diagnostic development. Beyond single reagents, we facilitate sourcing for validated antibody pairs, bulk ancillary reagents, and rare-target materials, streamlining your transition from concept to clinic. Whether you need custom monoclonal development or consistent lot-to-lot polyclonal supplies, our team bridges procurement gaps in the IVD supply chain.