Anti-GNB1 Polyclonal Antibody for WB, IF/ICC, ELISA - P62873

Core Product Specifications and Parameters

Biological Background: GNB1 Function and Localization

• GNB1 encodes the beta-1 subunit of heterotrimeric guanine nucleotide-binding proteins (G proteins), which act as modulators/transducers in various transmembrane signaling systems. Related references: PMID:29925951 PMID:33762731 PMID:34239069
• The beta and gamma subunits together are required for GTPase activity, GDP/GTP exchange, and G protein-effector interactions. Related references: PMID:29925951 PMID:33762731 PMID:34239069
• GNB1 belongs to the WD repeat protein family, characterized by multiple WD40 repeats that form a beta-propeller structure.
• The protein is subject to post-translational modifications including acetylation and phosphorylation.
• Mutations in GNB1 are associated with disease variants, including intellectual disability.
• Alternative splicing generates multiple transcript variants encoding different isoforms.
• GNB1 plays a role in host-virus interactions.
• Molecular weight: approximately 37.4 kDa (as per UniProt).

Experimental Guidance and Technical Tips

• For Western blot, the expected band size is around 37 kDa; use standard SDS-PAGE and transfer conditions.
• In immunofluorescence/ICC, consider fixation and permeabilization protocols suitable for membrane-associated proteins.
• The polyclonal nature of the antibody may recognize multiple isoforms; validate specificity in knockout or knockdown models if available.
• For ELISA, perform checkerboard titrations to determine optimal coating and detection antibody concentrations.
• Confirm cross-reactivity in mouse samples by pilot experiments, as reactivity has been validated in both human and mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This GNB1 polyclonal antibody supports research into G protein signaling and GPCR pathways. Beyond this antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your workflow.