Anti-GNAT3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - A8MTJ3

Core Product Specifications and Parameters

Biological Background: GNAT3 Function and Localization

• Guanine nucleotide-binding protein G(t) subunit alpha-3, also known as gustducin alpha-3 chain, is encoded by the GNAT3 gene and belongs to the heterotrimeric G protein alpha subunit family.
• It plays a prominent role in bitter, sweet, and umami taste transduction by coupling specific cell-surface taste receptors with a cGMP-phosphodiesterase, leading to a decrease in intracellular cAMP and cGMP levels and subsequent calcium influx. Related references: PMID:38600377 PMID:38776963
• Gustducin heterotrimer mediates responses to bitter and sweet compounds: the alpha subunit regulates phosphodiesterase activity, while the beta/gamma subunits activate phospholipase C, resulting in increased inositol trisphosphate and intracellular calcium.
• GNAT3 functionally couples to taste receptor heterodimers: TAS1R2/TAS1R3 for sweet sensation and TAS1R1/TAS1R3 for umami taste, as well as to T2R family bitter receptors such as TAS2R14. Related references: PMID:38600377 PMID:38776963
• In the gastrointestinal tract, GNAT3 acts as a lumenal sugar sensor in enteroendocrine cells, controlling Na+-glucose transporter SGLT1 expression and stimulating secretion of incretin hormones GLP-1 and GIP, thereby linking taste signaling to metabolic regulation and glucose homeostasis.
• Subcellular localization is cytoplasmic, with tissue-specific expression in taste buds of the circumvallate and foliate papillae, enteroendocrine L cells of the gut, and spermatozoa.
• Annotated molecular weight is approximately 40 kDa; post-translational modifications include myristoylation, which may facilitate membrane association.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60–130 of GNAT3 (NP_001095856.1), an internal region; the resulting polyclonal antibody is expected to recognize both native and denatured protein, suitable for applications such as WB, IF/ICC, and ELISA.
• For Western blotting, the predicted band is ~40 kDa; consider using appropriate blocking and primary antibody dilution based on endogenous expression levels in taste or intestinal tissues.
• In immunofluorescence/ICC, optimize fixation and permeabilization protocols as GNAT3 is a cytoplasmic protein; include appropriate negative controls and counterstaining.
• When performing ELISA, note that polyclonal antibodies may provide higher signal due to multi-epitope binding; titrate the antibody and use a matching secondary detection system.
• Confirm cross-reactivity with mouse and human samples; validate performance in your specific tissue or cell line of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive B2B sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. For targets like GNAT3 involved in taste transduction and metabolic regulation, we supply validated polyclonal antibodies, antibody pairs, and ancillary reagents in bulk. Whether you need optimized monoclonals, rare antigens, or one-stop procurement solutions, CamelBio streamlines your development pipeline with reliable, high-quality materials and technical consultation.