Anti-GNAT1 Rabbit Polyclonal Antibody for WB, ELISA - P11488

Core Product Specifications and Parameters

Biological Background: GNAT1 Function and Localization

• GNAT1 encodes transducin alpha-1 chain, the alpha subunit of the rod-specific heterotrimeric G protein that couples rhodopsin to cGMP-phosphodiesterase (PDE6) in the phototransduction cascade. Related references: PMID:22190596
• Functions as a GTPase molecular switch: inactive GDP-bound state is activated by light-stimulated rhodopsin, leading to GTP binding and subsequent activation of PDE6, which hydrolyzes cGMP and closes cation channels in rod outer segments.
• Required for normal scotopic (dim light) vision; loss-of-function mutations cause congenital stationary night blindness (CSNB) type 1G and CSNBAD3. Related references: PMID:22190596
• Predominantly expressed in rod photoreceptor cells of the retina, with lower expression detected in the ciliary body, iris, and retinal pigment epithelium. Related references: PMID:1614872, PMID:22190596
• Subcellularly localizes to photoreceptor outer segment and inner segment membranes, consistent with its role in the visual transduction machinery.
• Post-translational modifications include N-myristoylation, which facilitates membrane association, and phosphorylation, which modulates signaling activity.
• Contains conserved guanine nucleotide-binding and GTPase domains essential for nucleotide exchange and hydrolysis, common to the G protein alpha subunit family.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 58–178 of human GNAT1, a region likely solvent-exposed in the native protein and conserved across species, supporting reported cross-reactivity with mouse and rat.
• For Western blot, GNAT1 has a predicted molecular weight of ~40 kDa; use appropriate molecular weight markers and load retinal tissue lysates as positive controls.
• For ELISA, consider both direct and sandwich formats; as a polyclonal antibody, it may offer robust detection but lot-to-lot consistency should be verified.
• Given high sequence homology among G protein alpha subunits, cross-reactivity with related proteins (e.g., GNAI1, GNAI2) is possible; include appropriate controls when testing non-retinal samples.
• Ship on ice bags to ensure stability; upon receipt, aliquot and store at -20°C or -80°C and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers developing immunoassays targeting GNAT1 or investigating retinal disorders, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated supply chain and technical support help accelerate your diagnostic or research projects.