Anti-GNAS Rabbit Polyclonal Antibody for WB, ELISA - O95467 / P63092 / P84996 / Q5JWF2

Core Product Specifications and Parameters

Biological Background: GNAS Function and Localization

• GNAS is a complex locus that generates multiple transcripts through alternative splicing and alternative promoter usage; one product is neuroendocrine secretory protein 55 (NESP55), encoded by the O95467 isoform.
• NESP55 is a secreted proteoglycan that localizes to cytoplasmic and secretory vesicles.
• The protein undergoes post-translational cleavage on a pair of basic residues, a characteristic feature indicated by its keyword annotation.
• It is classified as a glycoprotein and proteoglycan, suggesting roles in extracellular matrix interactions or signaling.
• Mutations in the GNAS gene or aberrant splicing leading to altered NESP55 expression have been associated with Cushing syndrome, a disorder of cortisol excess.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region of human Gαs (P63092); antibodies raised against this peptide may recognize Gαs as well as other GNAS isoforms sharing this sequence, such as NESP55 and XLαs.
• In Western blot, multiple bands may be observed due to the various GNAS protein isoforms, with predicted molecular weights around 28 kDa, 44 kDa, 45 kDa, 77 kDa, 109 kDa, and 111 kDa. Validate band identity using appropriate controls or isoform-specific reagents.
• For ELISA, consider using the antibody as part of a sandwich pair; titrate both capture and detection conditions in your specific sample matrix.
• Cross-reactivity with mouse GNAS is reported; verify performance in mouse-derived tissues or cell lines if your experimental design requires it.
• Since this is a polyclonal antibody, lot-to-lot consistency should be monitored in critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-GNAS polyclonal antibody supports research into G protein-coupled signal transduction and neuroendocrine secretory pathways. As a single-source partner, CamelBio can also assist in sourcing rare target antibodies and optimizing reagent supply for large-scale assay development.