Anti-GNAQ Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50148

Core Product Specifications and Parameters

Biological Background: GNAQ Function and Localization

• Functions as a signal transducer downstream of G protein-coupled receptors (GPCRs), alternating between an inactive GDP-bound state and an active GTP-bound state (PubMed:37991948). Related references: PMID:37991948
• Activates phospholipase C-beta (PLC-beta) isoforms, catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate to produce the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:37991948). Related references: PMID:37991948
• Possesses low intrinsic GTPase activity, which converts bound GTP to GDP, thereby terminating the signal (PubMed:37991948). Related references: PMID:37991948
• Mediates FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed:27852822). Related references: PMID:27852822
• Subcellularly localized to the cell membrane, Golgi apparatus, nucleus membrane, and nucleus.
• Predominantly expressed in ovary, prostate, testis, and colon; down-regulated in peripheral blood lymphocytes of rheumatoid arthritis patients (at protein level).
• Classified as a transducer, GTP-binding protein, and hydrolase; subject to palmitoylation and ADP-ribosylation.

Experimental Guidance and Technical Tips

• The immunogen consists of the full-length human GNAQ protein (aa 1-359), enabling recognition of multiple epitopes by the polyclonal antibody.
• For Western blot (WB), a band at approximately 42 kDa is expected; validate the molecular weight in the relevant sample system.
• When using immunofluorescence (IF/ICC) or immunohistochemistry (IHC-P), consider optimizing fixation/permeabilization and antigen retrieval methods based on the specific tissue or cell type.
• The antibody cross-reacts with human, mouse, and rat GNAQ; verify species-specific reactivity if extending to other organisms.
• For ELISA applications, include appropriate positive and negative controls to ensure assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to a wide range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-GNAQ polyclonal antibody supports GPCR signaling and cellular signal transduction research. CamelBio also offers custom antibody development and rare target sourcing to help accelerate your project from concept to clinic.