Anti-GNAQ Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P50148

Core Product Specifications and Parameters

Biological Background: GNAQ Function and Localization

• GNAQ encodes the guanine nucleotide-binding protein G(q) subunit alpha, a transducer in GPCR signaling cascades. Related references: PMID:34556863, PMID:35672283, PMID:37991948
• The protein alternates between inactive (GDP-bound) and active (GTP-bound) states; GPCR activation promotes GDP release and GTP binding, while intrinsic GTPase activity returns it to the inactive state. Related references: PMID:37991948
• Activated GNAQ stimulates phospholipase C-beta (PLCB1-4), leading to production of second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Related references: PMID:37991948
• GNAQ is required for platelet activation, B-cell selection and survival, and chemotaxis of neutrophils and dendritic cells (by similarity).
• GNAQ transduces FFAR4 signaling in response to long-chain fatty acids. Related references: PMID:27852822
• Subcellular localization includes the cell membrane, Golgi apparatus, nucleus, and nuclear membrane.
• Predominantly expressed in ovary, prostate, testis, and colon; down-regulated in peripheral blood lymphocytes of rheumatoid arthritis patients (at protein level).
• Together with GNA11, required for heart development (by similarity).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50–150 of human GNAQ, a conserved region among human, mouse, and rat; consider verifying cross-reactivity in your target species and application.
• For Western blotting, consider using whole-cell lysates from tissues with high GNAQ expression (ovary, prostate, testis, colon) and validate with a loading control.
• For IHC-P, antigen retrieval may be necessary given the nuclear and multiple membrane localizations; check staining in positive and negative tissue controls.
• ELISA performance should be validated using recombinant protein or peptide to establish optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated anti-GNAQ monoclonal antibodies suitable for GPCR signaling research and assay development. Beyond individual reagents, we offer optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare target raw materials to accelerate your diagnostic pipeline.