Anti-GNAO1 Polyclonal Antibody for WB, IF-P, ELISA - P09471

Core Product Specifications and Parameters

Biological Background: GNAO1 Function and Localization

• GNAO1 encodes the G(o) alpha subunit, which functions as a transducer in G protein-coupled receptor (GPCR) signaling cascades.
  Related references: PMID:29925951 PMID:33408414 PMID:39103320

• The alpha subunit contains a guanine nucleotide binding site and alternates between an active GTP-bound state and an inactive GDP-bound state.
  Related references: PMID:39103320

• Upon GPCR activation, GDP is released and GTP binds, promoting signal transduction.
  Related references: PMID:39103320

• Intrinsic GTPase activity hydrolyzes bound GTP to GDP, terminating the signal.

• G protein signaling is modulated by regulatory proteins that influence GDP release and GTP hydrolysis.

• GNAO1 inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels.

• The protein is localized to the cell membrane, anchored by lipid modifications such as myristoylation and palmitoylation.

• Additional keywords include alternative splicing, ADP-ribosylation, disease variants in epilepsy, metal-binding, and GTP-binding.

Experimental Guidance and Technical Tips

• The immunogen targets a conserved region (amino acids 60–180) of human GNAO1, which likely contributes to the antibody’s cross-reactivity with mouse and rat orthologs. Consider validating specificity in the relevant experimental species.
• For Western blotting, the predicted molecular weight is approximately 40 kDa; use appropriate positive and negative controls to confirm detection in your sample system.
• In immunofluorescence (IF-P), permeabilization may be required to access the membrane-associated GNAO1 protein; optimize fixation and permeabilization conditions for your cell type.
• For ELISA applications, evaluate the antibody’s performance as a capture or detection reagent in your specific assay format, and consider pairing with a validated secondary antibody.
• As a polyclonal antibody, lot-to-lot consistency should be verified, especially in quantitative applications; inclusion of reference standards is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and research teams from early-stage discovery through clinical application. For G protein signaling targets like GNAO1, we offer a range of validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing options for rare or custom targets. Our portfolio is designed to streamline your assay development workflow, providing reliable raw-material supply with the flexibility to meet project-specific demands.