Anti-GNAI2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P04899

Core Product Specifications and Parameters

Biological Background: GNAI2 Function and Localization

• Guanine nucleotide-binding proteins (G proteins) act as transducers downstream of G protein-coupled receptors (GPCRs) in multiple signaling cascades. Related references: PMID:38505600, PMID:29925945, PMID:35637350
• The alpha chain contains the guanine nucleotide binding site and alternates between an active GTP-bound state and an inactive GDP-bound state. Related references: PMID:12359238
• GNAI2 inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, lowering intracellular cAMP, and activates NFAT transcription factor in endothelial cells to promote angiogenesis. Related references: PMID:38505600
• Plays an essential role in neutrophil recruitment during acute inflammation, involving both neutrophil and endothelial cell functions.
• Regulates the cell surface density of dopamine receptors DRD2 by sequestering them as an intracellular pool.
• Localizes to the cytoplasm, centrosome, and cell membrane.
• Highly expressed in endothelial cells.
• Belongs to the G-alpha family and is subject to ADP-ribosylation, myristoylation, and palmitoylation.

Experimental Guidance and Technical Tips

• The full-length immunogen (amino acids 1-355) suggests the antibody may recognize the native and denatured protein; consider optimizing conditions for each application.
• For western blotting, a band at approximately 40 kDa is expected; include appropriate positive and negative controls.
• In immunofluorescence/ICC, GNAI2 typically exhibits cytoplasmic and membrane localization; validate staining patterns in the relevant cell type.
• ELISA may be used for quantitative detection; pair with a suitable standard curve and verify linearity and sensitivity.
• Cross-reactivity with mouse and rat GNAI2 is confirmed by the supplier; further validation in the specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support diagnostic manufacturers and labs from concept to clinic. This anti-GNAI2 polyclonal antibody is relevant for research into GPCR signaling, inflammation, and angiogenesis, and our team can assist with tailored procurement solutions and technical consultation to streamline your development workflow.