Anti-GNAI2 Rabbit Polyclonal Antibody for WB, ELISA - P04899

Core Product Specifications and Parameters

Biological Background: GNAI2 Function and Localization

• GNAI2 encodes the guanine nucleotide-binding protein G(i) subunit alpha-2 (adenylate cyclase-inhibiting G alpha protein), a member of the G protein alpha subunit family that functions as a molecular switch in signal transduction.
• Couples to GPCRs such as ADORA1, CNR1, and FPR2, and alternates between active GTP-bound and inactive GDP-bound states; GPCR activation promotes GDP/GTP exchange. Related references: PMID:29925945 PMID:35637350 PMID:35365641 PMID:12359238
• Inhibits adenylate cyclase activity of ADCY1, ADCY5, and ADCY6, leading to decreased intracellular cAMP, and is involved in hormonal regulation of adenylate cyclase in response to beta-adrenergic stimuli.
• Plays a role in activating transcription factor NFAT in endothelial cells to promote angiogenesis, and is essential for neutrophil recruitment during acute inflammation (by similarity). Related references: PMID:38505600
• Regulates cell surface density of dopamine receptors DRD2 by sequestering them intracellularly.
• Predominantly cytosolic but also found at the centrosome and cell membrane; highly expressed in endothelial cells.
• Subject to lipid modifications (myristate and palmitate) and ADP-ribosylation, categorizing it as a lipoprotein and a transducer.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human GNAI2 protein (amino acids 1-355), which likely generates antibodies targeting multiple epitopes across the entire protein. When designing assays, consider that the antibody may recognize both denatured and native conformations, as the immunogen is a recombinant fusion protein.
• For Western blotting, validate in human and mouse cell lines or tissues known to express GNAI2, such as endothelial cells. A specific band at approximately 40 kDa should be detected; however, signal intensity may vary depending on sample preparation and expression levels.
• In ELISA applications, use recombinant GNAI2 protein or cell lysates as coating antigens; perform checkerboard titrations to determine optimal antibody and antigen concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting for GPCR signaling research and immunoassay development. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your projects from concept to clinic. Explore our GNAI2 antibody and complementary solutions for cell signaling and inflammation studies.