Anti-GNAI1 Rabbit Polyclonal Antibody for WB, ELISA - P63096

Core Product Specifications and Parameters

Biological Background: GNAI1 Function and Localization

• GNAI1 encodes the alpha subunit of the heterotrimeric G(i) protein, a transducer that couples GPCR activation to intracellular effectors by cycling between GDP-bound (inactive) and GTP-bound (active) states. Related references: PMID:18434541 PMID:8774883
• Upon activation, GNAI1 inhibits adenylate cyclase isoforms ADCY1, ADCY5, and ADCY6, reducing cAMP production and modulating downstream PKA-dependent pathways. Related references: PMID:8119955
• Beyond GPCR signaling, GNAI1 plays a critical role in mitosis: it ensures cytokinesis completion and recruits the dynein-dynactin complex to the cell cortex and centrosomes during metaphase. Related references: PMID:17635935 PMID:22327364
• GNAI1 localizes to multiple cellular compartments, including the plasma membrane, cytoplasm, nucleus, centrosome, and cell cortex, consistent with its diverse signaling and cell cycle functions.
• Post-translational lipidations at the N-terminus, such as myristoylation and palmitoylation, anchor GNAI1 to membranes and regulate its signaling activity.
• Clinically, GNAI1 mutations are linked to neurodevelopmental disorders including epilepsy and intellectual disability, underscoring its role in neuronal circuitry.
• The protein is also implicated in host-virus interactions, as several viral proteins target G protein signaling to hijack host cellular processes.

Experimental Guidance and Technical Tips

• The immunogen – a synthetic peptide spanning amino acids 50–150 of human GNAI1 – is designed to target a conserved region, supporting reactivity across human, mouse, and rat in WB and ELISA applications.
• For WB, expect a band at ~40 kDa; use whole-cell lysates or tissue extracts known to express GPCRs and G proteins (e.g., brain, HEK293).
• Since polyclonal antibodies may exhibit lot-to-lot variation, consider validating with a known positive control (e.g., cells treated with GPCR agonists or expressing recombinant GNAI1) when establishing assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop platform for IVD raw-material sourcing, offering validated antibodies, antigen pairs, and ancillary reagents to diagnostic manufacturers and researchers. This anti-GNAI1 polyclonal antibody supports the study of G protein-coupled receptor signaling – a pathway central to many diseases and drug targets. Through its global supplier network, CamelBio facilitates procurement of rare-target raw materials and bulk quantities, accelerating your development from concept to clinic.