Anti-GNA15 Rabbit Polyclonal Antibody for WB, ELISA - P30679

Core Product Specifications and Parameters

Biological Background: GNA15 Function and Localization

• Guanine nucleotide-binding protein subunit alpha-15 (GNA15), also known as G alpha-16 or GNA16, is a member of the G protein alpha subunit family that acts as a molecular switch in intracellular signaling cascades.
• In collaboration with the adapter protein TTC1, it promotes HRAS activation and ERK1/2 phosphorylation through a phospholipase Cbeta-independent mechanism. Related references: PMID:12748287 PMID:20639119
• It mediates EMR2 receptor signaling, resulting in Akt, MAPK, and NF-kappa-B pathway activation, which drives macrophage differentiation and inflammatory responses. Related references: PMID:28421075
• GNA15 is specifically expressed in hematopoietic cells and has been detected at the protein level in epididymis.
• The protein possesses GTP-binding and magnesium-binding domains, and it undergoes ADP-ribosylation, a key feature of G protein regulation.
• Identified in multiple proteomics studies and designated as a reference proteome component, underscoring its broad research relevance.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant protein fragment spanning amino acids 112-374, which includes the highly conserved GTP-binding domain; this design may enable recognition of the full-length native protein in western blotting.
• For western blot, consider using standard denaturing conditions and a validated detection system for proteins around 44 kDa; optimal antibody dilution should be determined empirically.
• The reported cross-reactivity with human and mouse suggests utility in common mammalian models; validate in the relevant sample system.
• ELISA applications may benefit from coating with the immunogen or purified native protein to confirm specificity; titration experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to validated IVD raw materials, including this anti-GNA15 antibody for GPCR signaling and hematopoietic research. In addition to monoclonal and polyclonal antibodies, we support bulk ancillary reagent procurement and can assist in sourcing rare-target reagents, ensuring your projects move efficiently from concept to clinical application.