Anti-GNA13 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14344

Core Product Specifications and Parameters

Biological Background: GNA13 Function and Localization

• GNA13 encodes the alpha subunit of heterotrimeric G proteins that act as key modulators and transducers in various transmembrane signaling systems. Related references: PMID:15240885, PMID:16705036, PMID:16787920
• It activates the small GTPase RhoA by binding and stimulating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF, and ARHGEF12/LARG), leading to downstream regulation of actin cytoskeleton and AP-1 transcription factor. Related references: PMID:12515866, PMID:15240885
• GNA13-dependent RhoA/ROCK signaling promotes tumor cell invasion and metastasis, highlighting its role in cancer progression. Related references: PMID:16705036, PMID:16787920, PMID:27084452
• It inhibits CDH1-mediated cell adhesion through a mechanism independent of Rho activation, affecting cell-cell contacts. Related references: PMID:11976333
• In lymphoid follicles, GNA13 transmits P2RY8- and S1PR2-dependent signals that inhibit germinal center B cell growth and migration outside the niche.
• Subcellularly, GNA13 is located at the cell membrane, melanosome, cytoplasm, and nucleus, consistent with its signaling versatility.
• It is highly expressed in testis, including Leydig cells and differentiating spermatogenic cells, and is detected in mature spermatozoa.
• The protein undergoes palmitoylation and phosphorylation, and alternative splicing generates multiple isoforms. It belongs to the G-alpha family and contains GTP/Mg2+ binding sites.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-210), which includes the GTPase domain. This conserved region likely contributes to cross-reactivity with mouse and rat orthologs.
• For Western blotting, expect a band at ~44 kDa. Validate the antibody in positive control lysates such as HeLa or Jurkat, and consider using a loading control for normalization.
• In immunohistochemistry (paraffin sections), test on human testis tissue where expression is abundant. Optimize antigen retrieval and antibody dilution for specific staining.
• For immunofluorescence/ICC, note that GNA13 displays both membrane and intracellular localization. Choose fixation and permeabilization protocols that preserve these structures.
• In ELISA applications, the antibody can be used for detection of recombinant or endogenous GNA13. Pair with an appropriate enzyme-conjugated secondary antibody and validate assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials, including this anti-GNA13 polyclonal antibody for G protein signaling and cancer biology studies. Our portfolio spans validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Contact us to bridge your project from concept to clinic with reliable supply and technical support.