Anti-GNA13 Rabbit Polyclonal Antibody for IHC-P - Q14344

Core Product Specifications and Parameters

Biological Background: GNA13 Function and Localization

• Serves as a guanine nucleotide-binding protein (G protein) that modulates various transmembrane signaling systems. Related references: PMID:15240885 PMID:16705036 PMID:16787920
• Activates RhoA by directly interacting with and stimulating Rho guanine nucleotide exchange factors (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF, ARHGEF12/LARG). Related references: PMID:12515866 PMID:15240885
• Downstream Rho signaling regulates transcription factor AP-1 and promotes tumor cell invasion/metastasis via the RhoA/ROCK pathway. Related references: PMID:16705036 PMID:16787920 PMID:27084452
• Inhibits CDH1-mediated cell adhesion independently of Rho activation. Related references: PMID:11976333
• In lymphoid follicles, transmits P2RY8- and S1PR2-dependent signals that restrict germinal center B cell growth and migration.
• Localized to the cell membrane, melanosome, cytoplasm, and nucleus.
• Highly expressed in testis, including Leydig cells and differentiating spermatogenic cells; altered expression in macrocephalic spermatozoa from infertile patients.
• Features GTP-binding sites, magnesium/metal-binding regions, palmitoylation, and phosphorylation; multiple isoforms generated by alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length GNA13 protein (aa 1–377), which may recognize multiple isoforms; verify specificity in your sample system.
• For IHC-P, standard antigen retrieval and blocking steps are recommended; optimization may be needed for high-expression tissues such as testis.
• Expected band sizes of 33 kDa and 44 kDa suggest possible isoform detection or post-translational modifications; validate by Western blot if needed.
• Cross-reactivity with mouse and rat indicates conserved epitopes; consider sequence alignment to confirm identity in these species.

CamelBio: Your One-Stop Sourcing Bridge

For developers studying G protein signaling, tumor metastasis, or germinal center biology, CamelBio serves as a comprehensive IVD raw-material sourcing partner. Beyond this GNA13 polyclonal antibody, CamelBio offers validated antibody pairs, optimized monoclonals, and bulk ancillary reagents to streamline your assay development. Our team also supports rare target raw-material sourcing, ensuring your IVD project from concept to clinic.