Anti-GNA13 Polyclonal Antibody for WB, IF/ICC, ELISA - Q14344

Core Product Specifications and Parameters

Biological Background: GNA13 Function and Localization

• GNA13 encodes the alpha-13 subunit of heterotrimeric G proteins, acting as a modulator and transducer in various transmembrane signaling systems. Related references: PMID:15240885 PMID:16705036 PMID:16787920
• GNA13 activates the small GTPase RhoA by directly binding and activating Rho guanine nucleotide exchange factors (RhoGEFs), including ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF, and ARHGEF12/LARG. Related references: PMID:12515866 PMID:15240885
• Through RhoA/ROCK signaling, GNA13 promotes tumor cell invasion and metastasis, with documented roles in cancer progression. Related references: PMID:16705036 PMID:16787920 PMID:27084452
• GNA13 inhibits CDH1-mediated cell adhesion via a mechanism independent of Rho activation. Related references: PMID:11976333
• In lymphoid follicles, GNA13 transmits signals downstream of P2RY8 and S1PR2, leading to inhibition of germinal center B cell growth and migration.
• Subcellularly, GNA13 localizes to the cell membrane, melanosomes, cytoplasm, and nucleus.
• GNA13 is highly expressed in testis, including Leydig cells and differentiating spermatogenic cells; its expression is altered in certain infertility conditions.
• Post-translational modifications include palmitoylation and phosphorylation; the protein binds GTP and magnesium ion, characteristic of its GTPase activity.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-210); this antibody may recognize both full-length and splice variants if the epitope is conserved.
• For Western blot, an expected band around 44 kDa is observed; testis lysates may serve as a positive control due to high GNA13 expression.
• When using immunofluorescence/ICC, consider fixation and permeabilization protocols that balance detection of membrane-associated and intracellular pools.
• Validate cross-reactivity in mouse and rat samples using appropriate tissue or cell lysates.
• For ELISA, titrate the antibody to determine optimal working concentration; note that technical validation data are not provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw-material supplier, providing one-stop sourcing for GNA13-related reagents, including validated antibody pairs, purified polyclonal antibodies, and ancillary buffers. Our portfolio supports signal transduction research, and we assist with bulk procurement and technical consultation to streamline your development pipeline.