Anti-GNA11 Rabbit Polyclonal Antibody (pAb) for WB, IF/ICC, and ELISA - P29992

Core Product Specifications and Parameters

Biological Background: GNA11 Function and Localization

• GNA11 encodes the guanine nucleotide-binding protein subunit alpha-11, also known as G(y) subunit alpha, a member of the heterotrimeric G protein alpha subunit family.
• It acts as a molecular switch in GPCR signaling: in the inactive state, it binds GDP; upon receptor activation, it exchanges GDP for GTP, shifting to an active conformation; its intrinsic GTPase activity then hydrolyzes GTP to GDP, terminating the signal. This cycle is tightly regulated by guanine nucleotide exchange factors and GTPase-activating proteins (PubMed:31073061). Related references: PMID:31073061
• Active, GTP-bound GNA11 directly stimulates phospholipase C-beta (PLCB1-4) to cleave PIP2, generating the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), which mobilize intracellular calcium and activate protein kinase C (PubMed:31073061). Related references: PMID:31073061
• GNA11 transduces signals from the long-chain fatty acid receptor FFAR4 (GPR120), linking nutrient sensing to cellular responses (PubMed:27852822). Related references: PMID:27852822
• In the respiratory epithelium, GNA11 relays OXGR1-dependent signals that trigger intracellular Ca2+ release and promote mucociliary clearance of inhaled pathogens.
• GNA11 localizes primarily to the cell membrane and is also found in the cytoplasm, consistent with its role as a peripheral membrane protein.
• Expression is notably detected in testis, suggesting tissue-specific functions in reproductive signaling.
• The protein is subject to post‑translational modifications including palmitoylation and ADP‑ribosylation, and mutations in GNA11 are associated with human diseases (keyword: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning residues 250–350 of human GNA11 (NP_002058.2), a region that may confer specific recognition of the GNA11 protein.
• When using this antibody for Western blot, a band at approximately 42 kDa is anticipated; confirm specificity in your sample matrix and consider loading controls.
• For immunofluorescence/ICC, GNA11 is expected to localize at the plasma membrane and in the cytoplasm; permeabilization and fixation conditions should be optimized for the cell type.
• Cross-reactivity is reported for human, mouse, and rat; validate in the relevant species and tissue to ensure signal fidelity.
• ELISA applications may benefit from titrating the antibody against the immunogen peptide or recombinant GNA11 as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents for GPCR and G protein signaling research. For GNA11-related assay development, we offer bulk antibody supply, matched detection pairs, and technical consultation to support your workflow from early-stage R&D to clinical application. Partner with us to secure high-quality raw materials for your signal transduction and disease biomarker programs.