Anti-GNA11 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P29992

Core Product Specifications and Parameters

Biological Background: GNA11 Function and Localization

• GNA11, also known as guanine nucleotide-binding protein G(y) subunit alpha, is a member of the heterotrimeric G protein alpha subunit family that acts as a molecular switch in signal transduction.
• The encoded protein cycles between an inactive GDP-bound state and an active GTP-bound state upon stimulation of G protein-coupled receptors (GPCRs), facilitating nucleotide exchange and signal propagation. Related references: PMID:31073061
• Active, GTP-bound GNA11 stimulates phospholipase C-beta (PLCB1-4) to hydrolyze phosphatidylinositol 4,5-bisphosphate, generating the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Related references: PMID:31073061
• GNA11 mediates signaling downstream of the free fatty acid receptor 4 (FFAR4) in response to long-chain fatty acids, linking nutrient sensing to cellular responses. Related references: PMID:27852822
• Together with the related protein GNAQ, GNA11 is required for embryonic heart development, as inferred from similarity data.
• GNA11 localizes primarily to the cell membrane and cytoplasm, with dynamic membrane association likely regulated by palmitoylation and other lipid modifications.
• In respiratory epithelium, GNA11 transduces OXGR1-dependent signals that lead to intracellular calcium release and promote mucocilliary clearance of airborne pathogens.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the helical domain of GNA11 (aa 250-350), which is highly conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• For Western blotting, expect a band at approximately 42 kDa; consider using reduced/denatured samples and validate with positive controls such as testis lysates or GPCR-stimulated cell lines.
• In IHC-P, optimize antigen retrieval conditions and antibody titration; initial testing on FFPE sections of human testis may help establish specific staining.
• For ELISA, include proper coating buffers, blocking steps, and specificity controls to minimize non‑specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your GPCR signaling studies, we supply validated GNA11 antibody pairs, optimized polyclonal antibodies like this product, bulk ancillary reagents, and assistance in sourcing rare targets. Our streamlined supply chain helps accelerate your assay development with reliable quality and regulatory support.