Anti-Gn1a Polyclonal Antibody for WB, ELISA - Q4ADV8

Core Product Specifications and Parameters

Biological Background: Gn1a Function and Localization

• Gn1a (OsCKX2) is a cytokinin dehydrogenase/oxidase (CKX) that catalyzes the degradation of cytokinins, plant hormones critical for cell division and development. This activity directly influences grain yield in rice by modulating inflorescence meristem activity (PubMed:15976269, PubMed:28337744). Related references: PMID:15976269 PMID:28337744
• The protein is a member of the FAD-dependent oxidoreductase family, containing a flavin adenine dinucleotide cofactor essential for its enzymatic function, and is classified as a glycoprotein.
• As a secreted protein, Gn1a localizes to the extracellular space, where it acts on extracellular cytokinins, impacting signaling in adjacent cells.
• Gn1a is predominantly expressed in vascular tissues of leaves, culms, inflorescence meristems, and flowers, aligning with its role in reproductive development.
• Functioning as a negative regulator of panicle branching, reduced Gn1a expression leads to enhanced cytokinin accumulation, promoting grain number (PubMed:15976269). Related references: PMID:15976269
• The protein's molecular weight is approximately 60 kDa, consistent with the observed 60 kDa on Western blots for the rice protein.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment encompassing amino acids 400-500 of rice Gn1a; the resulting polyclonal antibody may recognize linear and conformational epitopes within this region.
• For Western blotting, a specific band at ~60 kDa is expected; however, confirm the band pattern in your sample type, as post-translational modifications or splice variants may cause slight shifts.
• In ELISA applications, consider using the recombinant immunogen or purified native protein as a positive control, and validate against negative controls such as a null mutant or pre-immune serum.
• Cross-reactivity has been reported for Oryza sativa; for other plant species, sequence homology in the immunogen region should be checked before use. Perform an initial titration to determine the optimal antibody dilution for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to IVD raw materials and technical services. For plant biology and crop yield research, we supply validated antibodies like anti-Gn1a, along with complementary reagents, optimized antibody pairs, and bulk ancillary materials. Our platform streamlines sourcing of rare-target antibodies and supports assay development from concept to clinic, ensuring efficient procurement and technical consultation for your projects.