Anti-GMPS Polyclonal Antibody for WB, IP, ELISA - P49915

Core Product Specifications and Parameters

Biological Background: GMPS Function and Localization

• GMPS encodes GMP synthase [glutamine-hydrolyzing], also known as GMP synthetase or glutamine amidotransferase, a key enzyme in de novo purine biosynthesis.
• It catalyzes the glutamine-dependent conversion of xanthine monophosphate (XMP) to guanosine monophosphate (GMP), using ATP to form an adenyl-XMP intermediate.
• The enzyme is located in the cytoplasm, specifically within the cytosol.
• Alternative splicing generates multiple transcript variants.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or interactions.
• GMPS is classified as a ligase and belongs to the glutamine amidotransferase family; it is involved in purine metabolism and GMP biosynthesis.
• Chromosomal rearrangements involving this gene have been associated with certain cancers, and it is annotated as a proto-oncogene in UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 394–693 of human GMPS, a C-terminal region. Consider that this polyclonal antibody may recognize both full-length GMPS and any isoforms retaining this segment.
• For Western blotting, the predicted molecular weight is ~77 kDa. Validate band specificity by including positive and negative control lysates.
• The antibody is reported to cross-react with mouse and rat GMPS, making it suitable for comparative studies across these species. Verify reactivity in your specific sample matrix.
• For immunoprecipitation, optimize antibody concentration and incubation conditions, as polyclonal antibodies can yield higher background. Include an isotype control.
• In ELISA applications, consider using the recombinant immunogen as a positive control to confirm antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For GMPS-related studies—such as purine metabolism or oncology research—we offer one-stop sourcing and technical support to accelerate your assay development from concept to clinic. Contact us to explore custom bulk antibody solutions and rare target raw material sourcing.