Anti-GMNN Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75496

Core Product Specifications and Parameters

Biological Background: GMNN Function and Localization

• Geminin functions as an inhibitor of DNA replication by preventing the incorporation of the MCM complex into the pre-replication complex (pre-RC), a critical step in replication licensing. Related references: PMID:14993212 PMID:20129055 PMID:24064211
• Its cell cycle-dependent degradation during mitosis, occurring at the metaphase-anaphase transition, allows replication in the subsequent cell cycle. Related references: PMID:14993212 PMID:24064211 PMID:9635433
• Geminin inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, thereby suppressing histone H4 acetylation and further blocking DNA replication licensing. Related references: PMID:20129055
• It also negatively regulates the transcriptional activity of a subset of Hox proteins, integrating them into cell proliferative control. Related references: PMID:22615398
• The protein localizes to both the cytoplasm and nucleus, consistent with its roles in replication and transcription regulation.
• Post-translational modifications include phosphorylation and acetylation, which may modulate its stability and function.
• Mutations in the GMNN gene are associated with Meier-Gorlin syndrome 6 (MGORS6), a form of primordial dwarfism.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human GMNN protein (amino acids 1-209), suggesting the antibody can detect endogenous Geminin across multiple applications.
• For Western blotting, the predicted molecular weight is approximately 24 kDa; consider using appropriate gel percentages and transfer conditions.
• In IHC-P and IF/ICC, validate the antibody with appropriate controls and consider antigen retrieval methods for formalin-fixed tissues.
• Since Geminin is cell cycle-regulated, sample collection timing may impact detection; consider synchronizing cells or using inhibitors to enrich specific phases.
• The antibody cross-reacts with mouse GMNN, allowing use in common mouse models; however, optimal titers should be empirically determined for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for cell cycle and DNA replication research, including rare targets like Geminin. Explore our catalog for reliable raw-material sourcing to accelerate your assay development.